Quant-iT RiboGreen RNA Reagent is an ultrasensitive fluorescent nucleic acid stain for quantitating RNA in solution. The Quant-iT RiboGreen RNA Assay Kit comes with reagent, dilution buffer, and rRNA standards to provide a linear detection range of 1–200 ng of RNA.
Workflow
This easy-to-use reagent offers two detection ranges: a high-assay range and a low-assay range for sensitive and broad range detection. To use, simply prepare the working solution by diluting the provided dye with TE-Buffer, then aliquotting 100 µL of the working solution with standards and samples for a total reaction volume of 200 µL. RNA is then quantitated using a fluorescence microplate reader (such as Varioskan ALF) at standard fluorescein wavelengths.
